Which of the following is the most sensible order of techniques for mapping a chromosome and then locating a specific disease gene?
A)contig alignment;restriction mapping;RFLP linkage analysis;chromosomal walking
B)chromosomal walking;contig alignment;restriction mapping;RFLP linkage analysis
C)restriction mapping;chromosomal walking;contig alignment;RFLP linkage analysis
D)chromosomal walking;restriction mapping;RFLP linkage analysis;contig alignment
E)restriction mapping;contig alignment;RFLP linkage analysis;chromosomal walking

Question

Quizplus · Accepted Answer

Restriction mapping is often an initial step in chromosome mapping, providing a basic physical map. Contig alignment then helps in assembling a more detailed and comprehensive map. RFLP linkage analysis is useful for linking specific markers to disease traits. Finally, chromosomal walking is a technique used to move progressively across larger regions of a chromosome to pinpoint the location of a specific gene, such as a disease gene, making it a logical final step in this sequence.

Which of the Following Is the Most Sensible Order of Techniques