Given Below Are the Genotypic Frequencies for a Single Gene

Given below are the genotypic frequencies for a single gene with two alleles for three different populations.

$\begin{array}{llll} &\underline{A A} & \underline{A a}& \underline{a a}\\\text { Population 1 } & 0.25 & 0.50 & 0.25 \\\text { Population 2 } & 0.35 & 0.56 & 0.09 \\\text { Population 3 } & 0.49 & 0.42 & 0.09\end{array}$

Which of the following is/are NOT correct about these three populations?

A) One must assume Hardy-Weinberg equilibrium to calculate the allele frequencies.
B) Only two of the populations are in Hardy-Weinberg equilibrium.
C) Population 1 is in Hardy-Weinberg equilibrium;the frequency of allele A is 0.5.
D) Population 2 is not in Hardy-Weinberg equilibrium;the frequency of allele a is 0.37.
E) Population 3 is in Hardy-Weinberg equilibrium;the frequency of allele a is 0.3.