=NFE ^{$\circ$ '}; F
-An Important Reaction in the Production of Energy

=nFE ^{$\circ$ '}; F
-An important reaction in the production of energy from sugars (glycolysis) is the reduction of pyruvate to lactate by NADH.

pyruvate + NADH + H⁺(aq) $\to$ lactate + NAD⁺ $\Delta$ _rG ^{$\circ$ '}=?

Given the following reduction potentials,
NAD⁺ + H⁺ + 2e^- $\to$ NADH $~~~~~~~~$ E ^{$\circ$ '} = -0.320 V
Pyruvate + 2H⁺ + 2e^- $\to$ lactate $~~~~~~~~$ E ^{$\circ$ '} = -0.190 V

Calculate the standard Gibbs free energy (at pH 7) for the reduction of pyruvate to lactate by NADH.( $\Delta$ _rG ^{$\circ$ '}=nFE ^{$\circ$ '}; F = 96485 C/mol electrons)

A) 98.4 kJ/mol-rxn
B) -98.4 kJ/mol-rxn
C) -12.6 kJ/mol-rxn
D) 25.1 kJ/mol-rxn
E) -25.1 kJ/mol-rxn