The two dark lines in the trilaminar appearance of the plasma membrane are believed to be caused by the preferential staining of the hydrophilic polar regions of the membrane constituents.

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The dark lines in the trilaminar appearance of the plasma membrane are caused by the preferential staining of the hydrophilic polar regions of the membrane constituents, which are often rich in phospholipids with polar head groups. This leads to a three-layered appearance with two dark lines and a light central region.

The Two Dark Lines in the Trilaminar Appearance of the Plasma