Which of the Following Represents the Correct Order of Steps $\rarr$

Which of the following represents the correct order of steps usually taken in a Southern analysis?

A) electrophoresis $\rarr$ restriction digest $\rarr$ nitrocellulose transfer $\rarr$ hybridization
B) electrophoresis $\rarr$ hybridization $\rarr$ restriction digest $\rarr$ nitrocellulose transfer
C) hybridization $\rarr$ restriction digest $\rarr$ electrophoresis $\rarr$ nitrocellulose transfer
D) restriction digest $\rarr$ electrophoresis $\rarr$ hybridization $\rarr$ nitrocellulose transfer
E) restriction digest $\rarr$ electrophoresis $\rarr$ nitrocellulose transfer $\rarr$ hybridization