Which of the following describes the correct sequence of enzyme activity upon the lagging strand?
A)primase, DNA ligase, DNA polymerase I, DNA polymerase III
B)primase, DNA polymerase I, DNA polymerase III, DNA ligase
C)DNA polymerase III, primase, DNA polymerase I, DNA ligase
D)primase, DNA ligase, DNA polymerase III, DNA polymerase I
E)primase, DNA polymerase III, DNA polymerase I, DNA ligase

Question

Quizplus · Accepted Answer

The correct sequence of enzyme activity on the lagging strand is as follows: primase adds RNA primers, DNA polymerase III adds nucleotides to the 3’ end of the RNA primer forming the okazaki fragments, DNA polymerase I removes the RNA primer and replaces it with DNA, and finally, DNA ligase seals the nick between adjacent fragments by forming a phosphodiester bond. Therefore, the correct sequence is primase, DNA polymerase III, DNA polymerase I, and DNA ligase. Choice E is the only option that has this correct sequence.

Which of the Following Describes the Correct Sequence of Enzyme