How does the gp32 protein facilitate both stabilization of single-stranded DNA as well as its renaturation to double-stranded DNA?
A)gp32 is an enzyme and thus its reaction of separating DNA strands is freely reversible
B)gp32 binds only to regions of high A and T content so hydrogen bonding is reversible
C)gp32 displays cooperativity in binding to single stranded DNA
D)the gp32 C-terminal domain acts as a switch between weak and strong single stranded DNA binding
E)none of the above

Question

Quizplus · Accepted Answer

The gp32 C-terminal domain has been shown to act as a switch between weak and strong single-stranded DNA binding, allowing it to facilitate both the stabilization of single-stranded DNA and its renaturation to double-stranded DNA. This switch-like behavior likely arises from conformational changes in the protein induced by changes in the local DNA structure. The other options (A, B, and C) do not adequately explain how gp32 facilitates both single-stranded DNA stabilization and renaturation.

How Does the Gp32 Protein Facilitate Both Stabilization of Single-Stranded