Consider the two peptides shown below.Which of the following methods would be able to distinguish between these two sequences? Ala-Met-Val-Leu-Gly-Gly-Phe-Glu-Arg-Val
And
Ala-Val-Gly-Glu-Val-Met-Leu-Gly-Arg-Phe

Question

Quizplus · Accepted Answer

Chymotrypsin is a protease that cleaves amide bonds on the carboxyl side of aromatic residues (such as Phe). Therefore, it would cleave the first peptide between Phe and Glu, but not the second peptide. Edman's reagent and complete hydrolysis of all peptide bonds would not be able to distinguish between the two sequences.

Consider the Two Peptides Shown Below