The peptide Leu─Cys─Arg─Ser─Gln─Met is subjected to Edman degradation.In the first cycle the peptide first reacts with phenylisothiocyanate under basic conditions.The product of this reaction is incubated with anhydrous trifluoroacetic acid and subsequently with an aqueous acid.What are the products generated in the first cycle.

Question

Quizplus · Accepted Answer

Edman degradation specifically cleaves and labels the N-terminal amino acid of a peptide, resulting in a phenylthiohydantoin (PTH) derivative of the N-terminal amino acid and a shorter peptide. In this case, PTH-Leu is generated along with the remaining peptide Cys-Arg-Ser-Gln-Met.

The Peptide Leu─Cys─Arg─Ser─Gln─Met Is Subjected to Edman Degradation