Double stranded DNA molecules can be cleaved at specific recognition sites by

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Type II restriction endonucleases are enzymes that recognize specific DNA sequences and cleave them in a predictable manner. They are commonly used in molecular biology experiments for DNA manipulation and analysis, such as DNA cloning and sequencing. RNA polymerase, DNA ligase, DNA polymerase, and reverse transcriptase are all enzymes that play important roles in DNA replication, transcription, and repair, but they do not directly cleave double stranded DNA at specific sites.

Double Stranded DNA Molecules Can Be Cleaved at Specific Recognition