Which of the following methods will allow you to determine if a substance used mediated transport?

Question

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Evaluating the flux (rate of transport) at several different concentrations of the substance and looking for hyperbolic dependence is a common method to determine if a substance is transported via mediated transport. This type of transport exhibits saturation kinetics, meaning there is a limit to the rate of transport when all transporters are occupied. As concentration increases, the rate of transport will increase until all transporters are saturated, and the rate will plateau. This is seen as a hyperbolic curve when plotted.

Which of the Following Methods Will Allow You to Determine