In eukaryotes,which of the following would decrease the rate of transcription?
A)mutation in the nucleotide sequences at −10 and −35
B)mutation in the nucleotide sequences at −10 and +5
C)mutation in the nucleotide sequences at −70 and −90
D)mutation at the $\sigma$ binding site
E)all of the above

Question

In eukaryotes,which of the following would decrease the rate of transcription?
A)mutation in the nucleotide sequences at −10 and −35
B)mutation in the nucleotide sequences at −10 and +5
C)mutation in the nucleotide sequences at −70 and −90
D)mutation at the  $\sigma$ binding site
E)all of the above

Quizplus · Accepted Answer

The nucleotide sequences at −70 and −90 correspond to the enhancer and promoter regions that are crucial for transcription initiation. A mutation in these regions would impact the binding of the transcription factors and RNA polymerase, resulting in a decreased rate of transcription. Mutations at −10 and −35 or −10 and +5 would also impact transcription initiation, but to a lesser extent. Mutation at the F1F1F1S1F1F1F10 binding site would affect the binding of the RNA polymerase holoenzyme to the promoter, but it is not a commonly recognized binding site, hence not a likely scenario. Therefore, option C is the best answer.

In Eukaryotes,which of the Following Would Decrease the Rate of Transcription