Which of the following best describes the preparation of fMet-tRNA_f^met for translation in prokaryotes? A)Met is first formylated,then added to a tRNA specific for the start codon that differs from that designed to carry Met. B)Met is first added to a tRNA specific for the start codon that differs from that designed to carry Met,then is formylated. C)Met is first formylated,then added to a "regular" tRNA;the fact that it has the formyl group allows use in the initiation codon. D)Met is first added to a "regular" tRNA,then is formylated;the fact that it has the formyl group allows use in the initiation codon. E)f-Met isn't used in prokaryotes,only in eukaryotes.

Question

Quizplus · Accepted Answer

In prokaryotes, the N-formylmethionine (fMet) is first added to a tRNA specific for the start codon AUG that differs from the tRNA designed to carry regular methionine. This tRNA is recognized by the initiation factor 2 (IF2) and brings the fMet to the P-site of the ribosome to initiate translation. After initiation, the fMet-tRNAfMet is released from the P-site and normal elongation occurs with the recruitment of other tRNAs carrying regular methionine.

Which of the Following Best Describes the Preparation of FMet-TRNAfmet

Which of the Following Best Describes the Preparation of FMet-TRNA_f^met