What Would Be Important to Perform in Order to Obtain

What would be important to perform in order to obtain a good sputum sample that is to be used to detect the pathogen causing community-acquired pneumonia?

A) Ensure that antibiotic treatment was instituted at least 24 hr earlier.
B) Have patient sit in the semirecumbent position to obtain sample.
C) Have patient spit into sample container.
D) Patient should rinse mouth prior to sample collection.