Gram staining allows differentiation between bacterial cell types by:

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Gram staining is a method that differentiates between bacterial cell types based on the differences in their cell walls. Gram-positive bacteria have thick peptidoglycan layers in their cell walls, which retain the crystal violet stain used in the Gram staining process, making them appear purple under a microscope. In contrast, Gram-negative bacteria have thinner peptidoglycan layers but have an outer membrane containing lipopolysaccharides, which prevent the crystal violet stain from penetrating and cause them to appear pink or red after staining. Therefore, by observing the color and morphology of the stained bacteria under a microscope, we can differentiate between Gram-positive and Gram-negative bacteria. Measuring the zone of inhibition, determining the MBC, or allowing the bacteria to incubate for a designated time are not related to Gram staining.

Gram Staining Allows Differentiation Between Bacterial Cell Types By