During the production of polyclonal antibodies,how are the antigen-specific antibodies purified?
A) dialysis
B) gel electrophoresis
C) size exclusion chromatography
D) affinity chromatography

Question

Quizplus · Accepted Answer

Affinity chromatography is the method of choice for purifying antigen-specific antibodies during production of polyclonal antibodies. This is because it selectively binds antibodies that have high affinity for the antigen of interest, allowing for the purification of the specific antibodies from the mixture of polyclonal antibodies produced. Dialysis, gel electrophoresis, and size exclusion chromatography are not specific enough to purify only the antigen-specific antibodies.

During the Production of Polyclonal Antibodies,how Are the Antigen-Specific Antibodies