Which of the following is NOT a definition of fractional saturation for a system of [P] +[L] [PL]? A) [PL] / [PL] + [P] B) [PL] + [P] / [PL] C) [L] / [L] + K_d D) occupied binding sites / total binding sites

Question

Quizplus · Accepted Answer

Option A and D are correct definitions of fractional saturation. Option C is also correct, but it specifically applies to the Hill equation where the parameter K_d represents the concentration of ligand needed to reach 50% saturation. Option B, however, is not a correct definition of fractional saturation. The numerator is the total concentration of protein-ligand complex and free protein, while the denominator only includes the concentration of protein-ligand complex. In other words, it does not consider the unbound ligand concentration, which is a crucial factor in determining the extent of binding saturation.

Which of the Following Is NOT a Definition of Fractional