In the steady-state condition assumed in Michaelis-Menten kinetics,__________ is relatively constant. A) [ES] B) [S] C) v₀ D) [ES] / [S]

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In the steady-state condition assumed in Michaelis-Menten kinetics, the concentration of enzyme-substrate complex [ES] is relatively constant. At this point, the rate of formation of [ES] is equal to the rate of its breakdown, and the rate of product formation is at a maximum. The [S] concentration, on the other hand, may vary in different experimental conditions since it is the substrate that is being depleted by the reaction. The reaction velocity (v) is also not constant, but it approaches a maximum value (Vmax) when [S] is much higher than the Michaelis constant (Km), and [S] determines the initial rate of the reaction. [ES]/[S] is not necessarily constant, as it depends on both the reaction rate and the concentrations of [E] and [S].

In the Steady-State Condition Assumed in Michaelis-Menten Kinetics,__________ Is Relatively