A mixture of enzyme and inhibitor is run through a size-exclusion chromatography column.The activity of the enzyme is assessed before and after the chromatography.The enzyme has more activity after the chromatography step.Which of the following is true?
A) The enzyme was not eluted fully from the column.
B) The enzyme was denatured during chromatography.
C) The inhibitor is a reversible inhibitor.
D) The inhibitor is an irreversible inhibitor.

Question

Quizplus · Accepted Answer

The increase in enzyme activity after chromatography suggests that the inhibitor was separated from the enzyme, indicating that it is a reversible inhibitor.

A Mixture of Enzyme and Inhibitor Is Run Through a Size-Exclusion