What is the first step when analyzing glycan groups on a glycoprotein?
A) The glycan groups must undergo liquid chromatography to separate the different components of the mixture.
B) Mass spectrometry should be used to identify the glycans based on their mass to charge ratio.
C) The glycan groups must be separated from the protein moiety using a cleavage reaction.
D) The glycan groups must undergo column chromatography to separate the glycan groups from the lipid moiety.

Question

Quizplus · Accepted Answer

The first step when analyzing glycan groups on a glycoprotein is to separate the glycan groups from the protein moiety through a cleavage reaction. This allows for the carbohydrates to be analyzed separately from the protein and lipid components of the glycoprotein. Once separated, various analytical methods can be used for identification and characterization, such as liquid chromatography and mass spectrometry. However, these methods cannot be performed until the glycan groups have been cleaved from the protein. Therefore, option A, B, and D are incorrect.

What Is the First Step When Analyzing Glycan Groups on a Glycoprotein