In DNA sequencing,what is the purpose of the dideoxynucleotide bases?

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Dideoxynucleotides (ddNTPs) lack a hydroxyl (OH) group on the 3' carbon of their sugar ring, which is essential for the next nucleotide to be added during DNA synthesis. When incorporated into a growing DNA chain during sequencing, the lack of the OH group on the ddNTPs prevents further elongation of the chain, effectively stopping growth at a specific point. This creates a set of fragments of different lengths, which can be separated by size and read in order to determine the DNA sequence.

In DNA Sequencing,what Is the Purpose of the Dideoxynucleotide Bases