An immunoassay was performed in the following way: Antigen is bound to a solid support.Patient serum is added and patient antibodies bind to the antigen.The well is washed and enzyme-labeled anti-immunoglobulin antibodies are added.The well is washed and enzyme substrate is added and enzyme activity is determined.Which immunoassay is described?

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Quizplus · Accepted Answer

This immunoassay describes the ELISA (Enzyme-linked Immunosorbent Assay) method, which uses an enzyme-labeled secondary antibody to detect the presence of specific antibodies in patient serum. The antigen is bound to a solid support and patient antibodies bind to the antigen, forming an antigen-antibody complex. The addition of enzyme-labeled anti-immunoglobulin antibodies allows for the detection of this complex, and the enzyme substrate produces a color change that can be measured to determine enzyme activity.

An Immunoassay Was Performed in the Following Way: Antigen Is