How can the sensitivity of antibody detection be increased?

Question

Quizplus · Accepted Answer

All three options stated can enhance the sensitivity of antibody detection.

A) By increasing the ratio of patient serum to screening cells, the concentration of antibodies present for detection increases.

B) Extending the incubation time allows for more time for the antibodies to bind to the screening cells, thereby increasing the chance of detection.

C) The addition of potentiators such as albumin can improve detection by reducing non-specific binding and increasing the solubility of the screening cells, making them more accessible for antibody detection. 
Therefore, options A, B, and C can all enhance sensitivity, and therefore, option D is correct.

How Can the Sensitivity of Antibody Detection Be Increased