You Are Performing an IAT, and You Are Suspicious of the Results

You are performing an IAT, and you are suspicious of the results. It appears there may be a weak alloantibody present. You decide to repeat the test, and at the LISS stage you decide to add an extra two drops of serum to each tube being tested. What can you expect to happen?

A) There would be no effect.
B) The additional serum increases reaction strengths, because more possible antibody is added to the reaction.
C) Sensitivity of the test decreases, because you increased the ionic strength of the mixture.
D) You lowered the zeta potential, thus enhancing your results.