Bone marrow is going to be transplanted from a man into his sister. Which of the following laboratory procedures should be performed prior to this transplant to determine the HLA alleles of the donor and recipient at the highest resolution?

Question

Quizplus · Accepted Answer

Sequence-Based Typing (SBT) is the most accurate and high-resolution method for determining HLA alleles, which is important for bone marrow transplantation. Compared to other methods, such as the complement-dependent cytotoxicity (CDC) test or PCR-based techniques (PCR-SSP and PCR-SSOP), SBT provides unambiguous and detailed information about HLA alleles at the nucleotide level. This can help to identify potential mismatches between the donor and recipient, which may increase the risk of graft rejection or graft-versus-host disease. Therefore, SBT is the best choice for HLA typing in bone marrow transplantation.

Bone Marrow Is Going to Be Transplanted from a Man