Patient DNA has been mixed in separate wells with a probe that detects the 1691GA mutation associated with factor V Leiden and a probe that detects the normal sequence. Cleavase is added to all wells, and the fluorescence is measured in each well. Fluorescence was detected in the well that included the normal probe, and no fluorescence was detected in the well containing the mutant probe. Which of the following assays was performed?

Question

Patient DNA has been mixed in separate wells with a probe that detects the 1691G>A mutation associated with factor V Leiden and a probe that detects the normal sequence. Cleavase is added to all wells, and the fluorescence is measured in each well. Fluorescence was detected in the well that included the normal probe, and no fluorescence was detected in the well containing the mutant probe. Which of the following assays was performed?

Quizplus · Accepted Answer

The described procedure is characteristic of the Invader assay, which uses a Cleavase enzyme to cleave probes that are perfectly hybridized to their target DNA, resulting in fluorescence when the target matches the probe. The lack of fluorescence with the mutant probe indicates no match, while fluorescence with the normal probe indicates a match to the normal sequence.

Patient DNA Has Been Mixed in Separate Wells with a Probe