Which of the Following Enzyme/substrate Combinations Is Used in the Chromogenic

Which of the following enzyme/substrate combinations is used in the chromogenic detection of target sequences of nucleic acids after blotting procedures?

A) Horseradish peroxidase with Luminol/H₂O₂/p-iodophenol
B) Alkaline phosphatase with 1-2 dioxetane derivatives
C) Horseradish peroxidase with disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2'-(5'-chloro) tricyclo [3.3.1.1] decan}4-yl) -1-phenyl phosphate
D) Alkaline phosphatase with nitroblue tetrazolium and 5-bromo-4-chloro-3-indolyl phosphate