What will be observed in a PCR test of a true negative sample for the sample, positive control, negative control, and amplification control, respectively?

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Quizplus · Accepted Answer

In a PCR test, a true negative sample will not show amplification, hence it will be negative. The positive control, which contains the target DNA, should show amplification, indicating the PCR worked correctly, hence it is positive. The negative control, which does not contain any target DNA, should not show amplification, hence it is negative. The amplification control, which is used to confirm the PCR reaction worked correctly and is not dependent on the presence of the target DNA, should be positive.

What Will Be Observed in a PCR Test of a True