In the Sanger sequencing method, which of the following when incorporated into the growing strand are used to determine the identity of the base at a particular position in a piece of DNA?

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The Sanger sequencing method utilizes dideoxyribonucleotides (ddNTPs) in addition to the standard deoxyribonucleotides (dNTPs). These ddNTPs terminate the growing strand at specific positions, allowing for the identification of the identity of the base at that position. This is because the ddNTPs lack a 3' hydroxyl group, which is necessary for the formation of the phosphodiester bond that links nucleotides together. As a result, when a ddNTP is incorporated into the growing strand, it prevents the addition of any further nucleotides, effectively stopping DNA synthesis. The resulting fragments of different lengths can then be separated by gel electrophoresis and visualized to determine the DNA sequence.

In the Sanger Sequencing Method, Which of the Following When