Which of the Following Steps Can Be Taken to Minimize

Which of the following steps can be taken to minimize mis-priming in a PCR procedure?

A) Use high-quality deoxynucleotides, buffers, and enzymes.
B) Seclude the polymerase from the template and primers before amplification.
C) Load the samples carefully for gel electrophoresis of PCR products.
D) Keep the reaction mix at room temperature prior to amplification.