Which of the following is the correct order of the steps that occur in a standard PCR cycle?

Question

Quizplus · Accepted Answer

The standard PCR cycle always begins with a denaturation step that separates the double-stranded DNA template into single strands. The next step is annealing, during which the primers bind to the complementary regions on the template DNA. The final step is extension, during which the DNA polymerase synthesizes a new strand of DNA using the primers as a starting point. Therefore, the correct order of the steps is denaturation, annealing, and extension, which is only found in choice C.

Which of the Following Is the Correct Order of the Steps