Complementarity of primer pairs at the 3' ends will result in what artifacts?

Question

Quizplus · Accepted Answer

Complementarity of primer pairs at the 3F1F1F1S1F1F1F10 ends can result in the formation of primer dimers, which are non-specific amplification products formed by the annealing of the two primers. This can reduce the efficiency of the PCR reaction, leading to a decrease in the amount of specific product generated. Large mis-primed products may also form, but this is less common than the formation of primer dimers. No amplification may occur if the complementarity is too high, resulting in the formation of stable secondary structures that prevent the binding of the polymerase to the template DNA. 5F1F1F1S1F1F1F10 end degradation of the primers is not a common artifact associated with primer complementarity.

Complementarity of Primer Pairs at the 3' Ends Will Result