What determines the specificity in terms of the gene or region that is detected in the Southern blot?

Question

Quizplus · Accepted Answer

The labeled probe is designed to be complementary to a specific gene or region of interest, which determines the specificity of the detection in Southern blotting. The nitrocellulose membranes and agarose gel serve to allow for transfer and separation of DNA fragments, respectively, while restriction enzymes are used to cut the DNA at specific locations for analysis. However, the choice of probe is the main factor determining specificity.

What Determines the Specificity in Terms of the Gene or Region