A Southern blot procedure has been performed, but the molecular weight markers are the only bands seen on the gel. Apparently, the probe did not bind to its target on the membrane. How might the stringency be adjusted so that the probe will bind?

Question

Quizplus · Accepted Answer

Decreasing the incubation temperature reduces stringency, allowing the probe to bind more easily to its target sequence on the membrane.

A Southern Blot Procedure Has Been Performed, but the Molecular