A hybridization assay is run under the following conditions: 40% formamide with 0.5X SSC at 70^oC. When the bands were visualized, there were many more bands than expected in all wells on the membrane. Which of the following changes should be made to the hybridization conditions to get results that are closer to those expected?

Question

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Increasing the temperature will increase the stringency of the hybridization conditions, reducing non-specific binding and resulting in fewer, more specific bands.

A Hybridization Assay Is Run Under the Following Conditions: 40