A technologist is performing a total cell count on a synovial fluid. He prepares the undiluted fluid on a hemacytometer. An average of 213 cells was seen in 4 mm² of the chamber on each side of the hemacytometer. Calculate the total cell count.

Question

Quizplus · Accepted Answer

The formula for calculating the total cell count using a hemacytometer is: (average number of cells counted x dilution factor) / (area counted x depth of the chamber in mm). In this case, the dilution factor is 1 (undiluted), the area counted is 4 mm^2 (since 4 mm^2 were counted on each side), and the depth of the chamber is 0.1 mm. Therefore, the calculation is (213 cells x 1) / (4 mm^2 x 0.1 mm) = 532.5 cells/µL, which rounds to 533 cells/µL.

A Technologist Is Performing a Total Cell Count on a Synovial