Which cytochemical stain, more specific than SBB stain, should be recommended for granulocytic differentiation?

Question

Quizplus · Accepted Answer

Myeloperoxidase stain is commonly used for granulocytic differentiation as it specifically stains for the presence of myeloid cells. It works by reacting with the enzyme myeloperoxidase in the azurophilic granules of mature granulocytes. This stain is more specific than other cytochemical stains like nonspecific esterase or alpha-naphthyl butyrate. Specific esterase stain is useful for detection of monocytes and macrophages, while PAS stain is useful for identifying myeloblasts in acute myeloid leukemia.

Which Cytochemical Stain, More Specific Than SBB Stain, Should Be