When blood is inoculated into blood culture bottles using a winged blood collection set,the:

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The aerobic bottle should be inoculated first as it will allow for the detection of fast-growing aerobic organisms. Anaerobic bottle should be inoculated second as the oxygen in the aerobic bottle can affect the growth of anaerobic organisms. The safety device should be activated after collection to prevent accidental needle-stick injuries. The amount of blood inoculated should follow the recommended volume for blood culture collection to avoid under- or over-inoculation.

When Blood Is Inoculated into Blood Culture Bottles Using a Winged