The tourniquet is applied twice during the venipuncture procedure.

Question

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The tourniquet is usually applied once, before the venipuncture to enhance blood flow and make the veins more prominent. However, in some cases, such as when multiple vials of blood are being collected, the tourniquet may need to be released and reapplied to maintain adequate blood flow. In general, it is best to minimize the duration of tourniquet application to reduce the risk of hematoma formation or other complications.

The Tourniquet Is Applied Twice During the Venipuncture Procedure