In order to examine an opaque object with a compound microscope, one must:

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For examining opaque objects with a compound microscope, reflected illumination (also known as incident light) is used because the light reflects off the surface of the object into the microscope, allowing for visualization. Transmitted illumination (lighting the object from beneath) is not effective for opaque objects as they do not allow light to pass through them for viewing.

In Order to Examine an Opaque Object with a Compound