A Protein Contains Two Cysteine Residues That Are Widely Separated

A protein contains two cysteine residues that are widely separated in its amino acid sequence but form a disulfide bridge in its tertiary structure.Which is the most likely outcome if one of these cysteine residues is replaced with an alanine residue?

A) The tertiary structure will remain unchanged under normal physiological conditions and will also retain its stability under denaturing conditions.
B) The tertiary structure will remain unchanged under normal physiological conditions but will lose it ability to resist unfolding under denaturing conditions.
C) The tertiary structure will be disrupted slightly under normal physiological conditions but will retain its stability under denaturing conditions.
D) The tertiary structure will be greatly disrupted under normal physiological conditions but will retain its stability under denaturing conditions.
E) The tertiary structure will be greatly disrupted under normal physiological conditions and will lose it ability to resist unfolding under denaturing conditions.