Which of the following experimental designs can be expected to produce evidence that a membrane's fluidity is affected by temperature?
A) Collect fish of the same species from Lake Michigan in both winter and summer, and analyze differences in membrane protein composition of liver cells.
B) Compare the rate of intermixing of sheep and goat membrane proteins after fusing sheep and goat liver cells in a cold room and in a warmed incubator.
C) Isolate and analyze the fatty acids present in intestinal cell membranes from three different fish species from the Mediterranean Sea.
D) Hold fish of the same species at different environmental temperatures in a lab, then isolate their liver cells and analyze differences in their membrane proteins.
E) Compare the nucleotide sequences of the gene encoding the same receptor protein present in neuronal cell membranes from a desert mouse, Arctic rabbit, and tropical monkey.
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