The enzyme hexokinase catalyzes the reaction between ATP and glucose to form glucose 6-phosphate and ADP.In order to determine the efficiency of hexokinase, you wish to conduct an experiment to calculate its turnover number.To do so, you would set up

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To calculate the turnover number of an enzyme, it is necessary to measure the rate of the enzyme-catalyzed reaction at saturating substrate concentrations. The reaction also requires ATP as one of the substrates. Therefore, option C is the best choice as it involves a reaction with a known amount of enzyme and saturating levels of glucose and ATP to calculate the turnover number. Option A does not provide substrate saturation, option B only uses glucose 6-phosphate, and options D and E involve varying conditions that may affect the rate of the reaction differently.

The Enzyme Hexokinase Catalyzes the Reaction Between ATP and Glucose