Refer to the Figure Below, Which Shows the Reaction Between

Refer to the figure below, which shows the reaction between fumarate and water to produce malate. Fumarase is the enzyme that catalyzes this reaction in cells.Which statement can be used as evidence that orientation of reactant molecules is part of the function of fumarase as a catalyst?

A) Fumarase uses no coenzyme but requires one Fe²⁺ ion at its active site.
B) Fumarase catalyzes both the forward and reverse reactions between fumarate and malate.
C) Fumarase binds one fumarate molecule and one water molecule the same way for every reaction.
D) The active site of fumarase contains a histidine and a lysine that function as an acid and a base, respectively, during catalysis.
E) Mutation of the active site lysine to other amino acids obliterates or severely reduces activity in fumarase.