In an experiment, the F₁ portion of the ATP synthase was labeled with a fluorescently labeled microfilament and attached to a glass slide.The rotation of the ATP synthase was observed when ATP was added.When ATP plus oligomycin was added to the slide, the rotation continued even though oligomycin can inhibit ATP hydrolysis in mitochondrial inner membrane vesicles containing ATP synthase (F₁-F₀).Based on your knowledge of ATP synthase, which hypothesis is supported by these observations?

Question

Quizplus · Accepted Answer

Oligomycin's inability to inhibit ATP hydrolysis in the experiment suggests that its binding to ATP synthase is dependent on the enzyme's association with the membrane, as the rotation continued despite its presence. This implies that oligomycin requires a specific membrane-bound conformation of ATP synthase to exert its inhibitory effect.

In an Experiment, the F1 Portion of the ATP Synthase

In an Experiment, the F₁ Portion of the ATP Synthase