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Researchers Can Use PCR to Introduce Mutations in a Gene

Question 128

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Researchers can use PCR to introduce mutations in a gene.To do so, they can use a DNA primer that has one or more incorrect nucleotides.A low annealing temperature will allow the primer to anneal with the DNA and, if there are complementary base pairings at the 3ʹ end of the primer, initiate DNA polymerase activity.Why are these errors not corrected during PCR?

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