Refer to the figure, showing two culture plates. A researcher transfects bacteria with a gene that does not confer a selectable phenotype, so he also includes a gene for resistance to the antibiotic neomycin on a separate DNA strand.After the transfection, the researcher divides the culture into two equal fractions.Fraction 1 is spread onto a plate without neomycin (Plate A), and fraction 2 is spread onto a plate with neomycin (Plate B).Considering both plates, how many colonies in total are composed of bacteria that have been successfully transfected?
A) 15
B) 20
C) 5
D) 10
E) The total cannot be determined.

Question

Refer to the figure, showing two culture plates.  A researcher transfects bacteria with a gene that does not confer a selectable phenotype, so he also includes a gene for resistance to the antibiotic neomycin on a separate DNA strand.After the transfection, the researcher divides the culture into two equal fractions.Fraction 1 is spread onto a plate without neomycin (Plate A), and fraction 2 is spread onto a plate with neomycin (Plate B).Considering both plates, how many colonies in total are composed of bacteria that have been successfully transfected?
A) 15 
B) 20 
C) 5 
D) 10 
E) The total cannot be determined.

Quizplus · Accepted Answer

The Answer of Refer to the figure, showing two culture plates.  A researcher transfects bacteria with a gene that does not confer a selectable phenotype, so he also includes a gene for resistance to the antibiotic neomycin on a separate DNA strand.After the transfection, the researcher divides the culture into two equal fractions.Fraction 1 is spread onto a plate without neomycin (Plate A), and fraction 2 is spread onto a plate with neomycin (Plate B).Considering both plates, how many colonies in total are composed of bacteria that have been successfully transfected?
A) 15 
B) 20 
C) 5 
D) 10 
E) The total cannot be determined.

Refer to the Figure, Showing Two Culture Plates