What enables the specificity of PCR?
A) A heat-tolerant DNA polymerase
B) The use of particular buffers
C) The use of particular primers
D) The ratios of dNTPs used
E) The high temperature used to denature DNA

Question

Quizplus · Accepted Answer

The use of particular primers is what enables the specificity of PCR. Primers bind to the specific target sequence and initiate polymerization by the DNA polymerase.

Answer: A 
 A heat-tolerant DNA polymerase is also necessary for PCR as it can withstand the high temperature used to denature DNA, and can extend primers during the annealing step.

Therefore, the best choices are C and A.

What Enables the Specificity of PCR