Suppose the 5ʹ-to-3ʹ coding strand of DNA is GTCTATGCATTA. What is the template DNA strand that would be used for transcription?
A) 5ʹ-GTCTATGCATTA-3ʹ
B) 5ʹ-CAGATACGTAAT-3ʹ
C) 3ʹ-GTCTATGCATTA-5ʹ
D) 3ʹ-CAGATACGTAAT-5ʹ
E) 5ʹ-GUCUAUGCAUUA-3ʹ

Question

Quizplus · Accepted Answer

The template DNA strand is read in the opposite direction (3ʹ to 5ʹ) of the coding strand and complementary to it. Therefore, the template DNA strand for the given coding strand GTCTATGCATTA would be its complement read in the 3ʹ to 5ʹ direction, which is CAGATACGTAAT. Option D represents the template DNA strand in the 3ʹ to 5ʹ direction.

Suppose the 5ʹ-To-3ʹ Coding Strand of DNA Is GTCTATGCATTA