Suppose restriction enzyme A makes two cuts in a linear DNA fragment and restriction enzyme B makes one cut in that same DNA.The cuts are not in the same place, and no two of the cut fragments is exactly the same size.The digested samples are run in an electrophoresis gel.How many bands should the gel pick up if both enzymes A and B are used in the restriction digest?
A) Two
B) Three
C) Four
D) Five
E) Six

Question

Quizplus · Accepted Answer

Each cut by enzyme A will produce two fragments, and the cut by enzyme B will produce one fragment. Therefore, the total number of bands will be 2 x 2 + 1 = 5. As no two fragments are the same size, there will be five distinct bands on the gel.

Suppose Restriction Enzyme a Makes Two Cuts in a Linear